Useful Protocols for Life Scientists

Useful Protocols for Life Scientists

Ref: 10 Incredibly Useful Protocol Resources for Life Scientists | GoldBio

1.       Protocol Online - Index of Protocols (protocol-online.org)

Animal Techniques Angiogenesis Models, Animal Anesthesia, Animal Dosing, Animal Euthanasia, Animal Handling, Animal Histology and Pathology, Blood Collection from Small Animals, In Vivo Imaging, Misc. Animal Models, Pathology, Small Animal Identification Techniques, Surgery, Xenograft Tumor Models Biochemistry Amino Acids & Protein, Enzyme Analysis, Lipid Bioinformatics and Statistics Database Search, Sequence Analysis, Statistics Cell Biology Adhesion & Motility, Angiogenic Assay, Apoptosis, Cancer Cell Analysis, Cell Culture, Cell Cycle, Cell Growth & Cytotoxicity, Cell Invasion Assay, Cell Parts & Organelles, Cell Separation, Cell Synchronization, Cellular Staining, Chromosome, Cytology, Flow Cytometry (FCM), Metabolic Labeling, Senescence Assay, Signal Transduction, Stem Cells Developmental Biology Embryology, Fertilization, Microinjection Essential Data Amino Acids & Proteins, Centrifugation, Genetic Code, Molecular Weight Markers, Nucleic Acids, Restriction Enzymes, Vectors General Laboratory Techniques Autoradiography, Centrifugation, Isotope, Miscellaneous, Pipetting Techniques, Quantitation, Safety Procedures, Separation Techniques, Spectrophotometry, Sterile Technique, Water Treatment with DEPC Genetics & Genomics Cancer Genetics, Chromosome and Nucleosome Structure, Cytogenetics, Differential Display, Epigenetics, Gene Expression, Gene Therapy, Genetic Mapping, Genome Editing, Genotyping, Microarray, Microsatellite, SNP Analysis, Transgenic Techniques Histology Fixation, Microdissection, Microtomy, Microwave Techniques, Mounting, Plastic Embedding Techniques, Reagents & Solutions, Sectioning, Specimen Preparation, Staining, Tissue Microarray (TMA) Imaging Techniques Microscopy, Radiologic Imaging Immunology Antibody, Antigen, Bromodeoxyuridine (BrdU) Staining, Chemotaxis, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Macrophages & Monocytes Media & Solutions Microbiology Bacteria, Fungi, Virus Model Organisms Arabidopsis, C. Elegans, Drosophila, Rat & Mouse, Xenopus, Yeast, Zebrafish Molecular Biology Carbohydrate, Cosmid, DNA, DNA Sequencing, DNA-Protein Interactions, Electrophoresis, Enzymatic Manipulation of Nucleic Acid, Molecular Cloning, Oligonucleotide, PCR, Phage, Plasmid, Promoter Cloning, Characterization and Transcription Analysis, Protein, RNA, Transfection, Transformation Neuroscience Neuroanatomy, Neurochemistry, Neurophysiology Pharmacology & Toxicology Physiology Hematology, Metabolic Analysis Plant Biology PCR, Photosynthesis, RNA, Tissue Culture Research Tools Online Tools, Software

2.       Nature Protocols

Note: it needs registration/subscription.

3.       Springer Protocols platform has migrated to Experiments (springernature.com)

Note: it needs registration/subscription.

4.       JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

Note: it needs registration/subscription.

5.       OpenWetWare

Note: is an effort to promote the sharing of information, know-how, and wisdom among researchers and groups who are working in biology & biological engineering

6.       Bio-protocol - Improve Research Reproducibility (bio-protocol.org)

Note: it needs registration/subscription.

7.       SciGine - Scientific Methods for Biology & Biochemistry

Note: free available.

8.       Protocols and troubleshooting tips (abcam.com)

Note: free available.

Protocols and troubleshooting tips on how to get the best from our antibodies

·         Antibody storage guide

·         Need help or want to give feedback on our protocols? Contact Abcam's Scientific Support Team

Protocols - by application

Antibody conjugation and purification (2)+

Cell culture (7)+

Cell isolation (1)+

ChIP (7)+

Dot blot (1)+

ELISA (10)+

ELISPOT (2)+

Flow cytometry / FACS (13)+

Immunoprecipitation (7)+

Immunostaining (19)+

siRNA  (1)+

Toxin neutralization (2)+

Western blot (17)+

Protocols - by research area

Apoptosis protocols (5)+

Cardiovascular protocols (3)+

Molecular biology protocols (5)+

Neuroscience protocols (13)+

Stem cells protocols (3)+

Protocol guides and diagrams

Detailed application guides (4)+

Protocol diagrams (8)+

General help

Frequently asked questions (5)+

Product guides (5)+

Secondary antibody and fluorescence guides (6)+

Troubleshooting tips (7)

9.       Protocols | Thermo Fisher Scientific - CA

Note: free available.

Nucleic Acid Purification & Analysis

Agarose Protocols | DNA Analysis Protocols | DNA Extraction Protocols | mRNA Protocols | RNA Protocols

Nucleic Acid Amplification & Expression Profiling

cDNA Protocols | DNA Extraction Protocols | PCR Protocols

Cloning

Cloning Protocols | Competent Cell Protocols | Ligation Protocols | Transformation Protocols

Proteins, Expression, Isolation & Analysis

Adenovirus Protocols | Antibody Protocols | Cell Separation Methods | GFP Protocols | Immunoprecipitation Protocols | Membrane Protein Protocols | Protein Expression Protocols | Protein Isolation Protocols | Protein Purification Protocols | SDS PAGE Protocols | Western Blot Protocols | T Cell Activation & Expansion Protocols | ELISA Protocols

RNAi, Epigenetics & Gene Regulation

RNAi Protocols | siRNA Protocols

Cell Culture

3D Cell Culture Protocols | Cell Culture Protocols | CHO Cell Culture Protocols | Neural Cell Culture Protocols | MTT Assay Protocols | Primary Cell Protocols | Serum Free Media Protocols | Stem Cell Protocols | Transfection Protocols

Cell Analysis

Cell Proliferation Protocols | Cell Viability Protocols | Cell Structure Protocols | Microscopy Protocols | Flow Cytometry Protocols | High-Content Screening (HCS) Protocols | Microplate Protocols

Drug Discovery & Development

ADME/Tox Protocols

Immunology

Immunology Protocols

Neurobiology

Neurobiology Protocols | Neural Cell Culture Protocols | Neural Stem
Cell Protocols

Stem Cell Research

Pluripotent Stem Cell Protocols | Neural Stem Cell Protocols | General Stem Cell Protocols

10.   https://www.sigmaaldrich.com/life-science

Note: free available.

Biochemicals & Reagents

• Amino Acids
• Antibodies
• Antibiotics
• Biological Buffers
• Carbohydrates
• Detergents
• Enzymes, Inhibitors, and Substrates
• Life Science Labware
• Lipids
• Peptides
• Proteins & Derivatives
• Stable Isotopes
• Additional Biochemicals & Reagents

Techniques & Applications

·       Automation Reagents

·       Cell Culture

·       Cell and Cell-Based Assays

·       Chromatography

·       Cloning & Expression

·       Detection

·       Electrophoresis

·       Hematology & Histology

·       Mass Spectrometry

·       Nucleic Acid Purification

·       PCR & Purification

·       Post-Trans. Modification

·       Protein Purification & Detection

·       Super-Resolution Microscopy

Areas of Research

·       Cancer Research

·       Cell Biology/Cell Signaling

·       Drug Discovery

·       Epigenetics

·       Functional Genomics & RNAi

·       Metabolomics

·       Microbiology

·       Molecular Biology

·       Nutritional Research

·       Obesity Research

·       Plant Biotechnology

·       Proteomics

·       Stem Cell Biology

·       Synthetic Biology