Jinjiang: November 2012

IHC World

Protocol Database

Ref: http://www.ihcworld.com/general_IHC.htm

General IHC Protocols

· Blocking of Unwanted Non-specific Staining

· Immunoenzyme (HRP) Method

· Immunoenzyme (AP) Method

· Immunofluorescence Method

· Standard ABC (Avidin-Biotin Complex) Method

· Standard LSAB (Labeled StreptAvidin Biotin) Method

· Labeled Polymeric Methods

· Polymer Enhanced Method - A Highly Sensitive IHC Detection Protocol

· CSA (Catalyzed Signal Amplification) Method

· Immunohistochemical Staining Method for Mouse Antibody on Mouse Tissue

· Isotype-specific Immunostaining with Mouse Primary Antibodies on Mouse Tissue

· Immunohistochemistry Protocol on Mouse Spine

· Double Immunohistochemistry Staining Protocol

· Double Immunofluorescence Staining for Bcl-6 and else

· Immunohistochemistry Double Staining Method

· Immunoflurescence Double Staining Method - Parallel Approach

· Immunofluorescence Double Staining Method - Squential Approach

IHC Protocols from R&D Systems

· Fluorescent ICC Staining of Cells on Coverslips

· Fluorescent ICC Staining of Non-adherent Cells

· Chromogenic IHC Staining of Paraffin-embedded Tissue Section

· Chromogenic IHC Staining of Frozen Tissue Sections

· Fluorescent IHC Staining of Paraffin-embedded Tissue Sections

· Fluorescent IHC Staining of Frozen Tissue Sections

· Heat-induced Epitope Retrieval (HIER)

· Making a 4% Formaldehyde Solution in PBS

· Gelatin-coated Slides for Histological Tissue Sections

IHC Double Staining Techniques and Protocols

· Double-label Fluorescent Immunohistochemistry with Identical Species-derived Primary Antibodies.

· Double immunofluorescent staining using two unconjugated primary antisera raised in the same species

· A simple method for immunofluorescent double staining with primary antisera from the same species

· Microwaving for double indirect immunofluorescence with primary antibodies from the same species and for staining of mouse tissues with mouse monoclonal antibodies

· A method for double immunofluorescent staining by the indirect procedure with antibodies of the same isotype

· Improved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence

· An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo- and chlorodeoxyuridine incorporated into DNA

· Double staining technique using a combination of indirect and direct immunofluorescence with monoclonal antibodies

· Immunohistochemical double staining of microwave enhanced and nonenhanced nuclear and cytoplasmic antigens

· Immunohistochemical double staining with immunogold-silver and alkaline phosphatase to identify nuclear markers of cellular proliferation

· Immunohistochemical double-staining of renal allograft tissue: critical assessment of three different protocols

· A new double labeling technique for combined in situ hybridization and immunohistochemical analysis

IHC Triple Staining Techniques and Protocols

· A triple-labeling method: HRP anterograde tract tracing combined with double immunofluorescent cell staining in developing neural tissue of the rat

· Triple Immunofluorescence Staining with Antibodies Raised in the Same Species to Study the Complex Innervation Pattern of Intrapulmonary Chemoreceptors

· Light microscopic triple-colored immunohistochemical staining on the same vibratome section using the avidin-biotin-peroxidase complex technique

· An immunoenzyme triple-staining method using both polyclonal and monoclonal antibodies from the same species. Application of combined direct, indirect, and avidin-biotin complex (ABC) technique

· Triple labeling with two-color immunofluorescence using one light source: a useful approach for the analysis of cells positive for one label and negative for the other two

· Three-color immunofluorescence histochemistry allowing triple labeling within a single section

· Triple immunological staining with colloidal gold, fluorescein and rhodamine as labels

· Rapid triple-labeling method combining in situ hybridization and double immunocytochemistry

· Triple staining of normal and degenerating nervous tissue

· Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electron microscopical double and triple staining method employing primary antibodies from the same species

· Discriminative staining methods for the nervous system: luxol fast blue--periodic acid-Schiff--hematoxylin triple stain and subsidiary staining methods

Application of IHC Double Staining Techniques and Protocols

Application of IHC Triple Staining Techniques and Protocols

Protocols and troubleshooting tips

Protocols - by application

Ref: http://www.abcam.com

Antibody conjugation and purification (2)+

Sodium Azide removal protocol

Biotin conjugation

Cell culture (6)+

Cell isolation (1)+

T cell purification from splenocytes

ChIP (7)+

Dot blot (1)+

Dot blot protocol

ELISA (12)+

ELISPOT (3)+

Flow cytometry / FACS (13)+

Immunoprecipitation (6)+

Immunostaining (21)+

siRNA (1)+

Recommended checks and controls for siRNA experiments

Toxin neutralization (2)+

Western blot (17)+

Western blotting - a detailed guide

Protocols - by research area

Apoptosis protocols (5)+

Cardiovascular protocols (3)+

Molecular biology protocols (4)+

Neuroscience protocols (11)+

Protocol guides and diagrams

Detailed application guides (3)+

Protocol diagrams (8)+

General help

Frequently asked questions (6)+

Product guides (5)+

Protocols book (1)+

Limited edition protocols book

Secondary antibody and fluorescence guides (6)+

Troubleshooting tips (8)

Jinjiang

Friday, 16 November 2012

Jinjiang's WebWatcher on Biology (11) - Online immunohistochemistry

Protocols - by application

Ref: http://www.abcam.com

Protocols - by research area

Protocol guides and diagrams

General help

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